Nanopocket Membrane Fabrication-Update

Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely that we will have the word”early” from our manuscript title. PS: The old, raw mock…

Introduction Throughout my rotation, I have been working on the dialysis project with Alec. The goal was to set up a multi-pass system to analyze the membrane’s ability to separate different molecules in blood. The critical separation is between β2-microglobulin (12 kDa) and albumin (66 kDa) – β2-microglobulin is a large toxin that needs to…

The older sepcon macro has been update to fix previous issues and yield more meaningful results. These updates are being brought up to get feedback from the group and from there implement them and make these changes to the newer sepcon patterns and eventually the TEM patterns. Gathering and analyzing statistical data is standard practice…

W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….

Here are my slides for presentation at ICNMM. I’m linking them here to facilitate discussion at NRG later today, if desired. .pptx version – ICNMM2016 Presentation Slides .pdf version – ICNMM2016 Presentation Slides

One of the themes in my recent posts has been the presence of ‘vacuoles’ over free-standing pnc-Si and PET membranes. We’ve assumed these are vacuoles because endothelial cells form vacuoles, they don’t stain with live cell stains (and so have no cytoplasmic enzymes) and there can be several of these structures in each cell.  In…