SepCon® Video Protocol: Wetting the membrane
Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.
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SEPCON assembly – written description
Following is a written protocol for SEPCON assembly. (Here is a link to a video demonstration) Materials required: wafer tweezers, small sample tweezers (preferably plastic), soft foam board from craft store, wafer of sepcon chips, razor blade, plastic enclosure to store complete SEPCON, top basket, bottom basket, 300 µm thick silicone sheet of pre-cut “U”…
Importing Gasket Geometries to Silhouette from Sketchup
We currently use Adobe Illustrator to design silicone gasket geometries for our microfluidic devices. Here is a quick way to do a similar thing from sketchup. The advantage of this procedure is that you can create a complete 3d model of your device and export different snapshots to the 3d printer for bulk fabrication and a…
Immunofluorescence staining of junctional proteins and adhesion molecules of hiPSC-derived EECM-BMEC-like cells on nanomembranes
I would like to share my successful immunofluorescence stainings of adhesion molecules and junctional proteins of hiPSC-derived brain microvascular endothelial cell (BMEC)-like cells on nanomembranes. The cells were differentiated by the extended endothelial cell culture method (EECM). You can find detailed information about EECM-BMEC-like cells in the previous post submitted by Hideaki Nishihara (24.10.2020). Method…
Protocols of cell culture and seeding cells on CytoVu
I got some experiences in dealing with cells. This is for your reference. Aseptic Technique and Good Cell Culture Practice Sanitize the cabinet using 70% ethanol before commencing work. Sanitize gloves by washing them in 70% ethanol Put all materials and equipment into the cabinet prior to starting work after sanitizing the exterior surfaces with…