Protocols (Public)

I would like to share my successful immunofluorescence stainings of adhesion molecules and junctional proteins of hiPSC-derived brain microvascular endothelial cell (BMEC)-like cells on nanomembranes. The cells were differentiated by the extended endothelial cell culture method (EECM). You can find detailed information about EECM-BMEC-like cells in the previous post submitted by Hideaki Nishihara (24.10.2020). Method…

Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.

The following procedure is a first draft of the procedure used by Jim and I in an attempt to label BSA with TRITC. This is likely to be updated with photos of the procedure in the future. Prepare reaction buffer by dissolving 1.7g sodium bicarbonate in 200mL of water for a 100mM concentration. Begin to…

Following is a written protocol for SEPCON assembly.  (Here is a link to a video demonstration) Materials required: wafer tweezers, small sample tweezers (preferably plastic), soft foam board from craft store, wafer of sepcon chips, razor blade, plastic enclosure to store complete SEPCON, top basket, bottom basket,  300 µm thick silicone sheet of pre-cut “U”…

SEPCON assembly

I got some experiences in dealing with cells. This is for your reference.  Aseptic Technique and Good Cell Culture Practice  Sanitize the cabinet using 70% ethanol before commencing work.  Sanitize gloves by washing them in 70% ethanol  Put all materials and equipment into the cabinet prior to starting work after sanitizing the exterior surfaces with…

I’ve been working over the holiday on trying to make Ag/AgCl electrodes.  Here’s the method I’ve come up with so far: 1. 99.99% silver wire is used as the anode (+) and platinum as the cathod (-). 2. Silver wire is coated with chloride in a 1M HCl solution at a constant current of 10…