Introduction Pericytes are mural cells that line the outer surface of microvessels and regulate diverse aspects of vascular development and function. We differentiate brain-pericyte-like cells (BPLCs) from hiPSC clones using the protocol published by Eric Shusta’s lab (Gastfriend et al., 2021) (Figure 1). This protocol describes methods to differentiate hiPSCs to BPLCs via neural crest…
I would like to share my successful immunofluorescence stainings of adhesion molecules and junctional proteins of hiPSC-derived brain microvascular endothelial cell (BMEC)-like cells on nanomembranes. The cells were differentiated by the extended endothelial cell culture method (EECM). You can find detailed information about EECM-BMEC-like cells in the previous post submitted by Hideaki Nishihara (24.10.2020). Method…
20210122.flow-movie-demo I attached a link to a short presentation regarding the flow movie setups. The figures and videos will be explained in detail during the meeting on Friday.