Flow demo (T cell interaction with human in vitro BBB model under flow)
I attached a link to a short presentation regarding the flow movie setups. The figures and videos will be explained in detail during the meeting on Friday.
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Micro three-way valve http://www.theleeco.com
#LFYA1226032H 12volts 0.054inch ports(push on tubing)& deadspace=22uL
You do need to make an electronic box- I had one made by our department’s electronics guy.
Thanks for showing us your system- Always interested in seeing the clever ways people do this stuff!
Coisne, C., R. Lyck, and B. Engelhardt, Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo. Fluids Barriers CNS, 2013. 10(1): p. 7.
I upload pdf file of this paper and recorded zoom meeting in media.