These are the sieving curves for 50, 75, and 100 nm NPN membranes. The 75 nm NPN 30 nm gold data point is just an estimate, since the membrane broke before enough filtrate could be collected for an absorbance measurement. However, our estimates of zero gold tend to be pretty good (gold is very visible in solution,…
Hey all, Here’s my thesis: ksmith thesis I filmed the public portion of my defense and posted the video to youtube. I’m not leaving till the end of the week, and I’ll be back for a week in mid-May as well as being generally around Rochester this summer (this isn’t goodbye!) but my permanent email address…
The x-values are based on TEM images of the last populations of gold nanoparticles (I think. I can’t remember immediately when the fridge died on us). It’s probably worth re-doing that with the new gold vials. There’s still some work to be done. In the 50 nm NPN, 5 nm gold was only done once,…
For the modeling paper, I had to precisely quantify the distribution of gold nanoparticles in the BBI solutions. To do this, I dried the stock solutions of particles on TEM grids (I don’t know what chips I used, but I seem to recall – and take this with a grain of salt – that the grids…
This blog post details how to assemble a variant of Jirachai’s microfluidic device (which looks like this): From its constituent parts (mostly this, two separate devices shown): Cutting the Gaskets You can find the template used to cut the 6 layers in Jirachai’s folder on the desktop mac in the computing alcove downstairs. The file…
We’re submitting the following paper to Separations and Purifications within the next two weeks: model-paper model supplement Below are the (almost) final versions of the figures from the paper. Figure 1: Schematic of Particle Distribution for Particles Rejected by Membranes The distribution is a quasi-equilibrium between convection towards the membrane and back diffusion, which is…
Photo Courtesy J. Adam Fenster, U of R We’ve had several of each of the two prototype reverse Sepcon designs professionally 3D printed for us (top photo). I did a few quick tests to see how they worked in the lab. “Fixed” Version This is designed to snap onto the outer lip of a 2…
We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using reverse centrifugation: The earlier work used Josh Winan’s jury-rigged PDMS-filled reverse centrifugation setup, but despite the…
Below are drafts of all the figures I am planning on incorporating into my upcoming model paper. I believe I am done with data collection at this point. Data in figures not marked “NEW” probably needs to be updated. Note that all model curves are calculated with no fitting parameters. They are derived from base principles. That’s why…
It has been a constant theme in our EO efforts as well as our energy harvesting efforts that our electrodes need to be as close as possible to the surface to see an effect. In Jess’s early EO work, we found that our chips could be used as EO pumps that were vastly more thermodynamically…