Hey all,
Here’s my thesis: ksmith thesis
I filmed the public portion of my defense and posted the video to youtube.
I’m not leaving till the end of the week, and I’ll be back for a week in mid-May as well as being generally around Rochester this summer (this isn’t goodbye!) but my permanent email address will be karljpsmith@gmail.com.
Keep the dream alive!
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…
I’ve received some of the very first MgF2 material that I produced way back when and shipped off to Nottingham. These chips were produced from NPN wafer 1085 (5-slots). There are a few membranes still remaining, but mostly the chips are in tatters. Tomogram Reconstruction Weighted Back Projection (quick and dirty) We…
All separations are 30 nm (diameter) gold nanoparticles in a 56 nm pore setup, with other parameters as described in my last post. Y-axis scale bar is in meters, x-axis scale is meaningless (the simulation is 1-D, and the width is added just for clarity’s sake). The membrane is at y = 0. Color corresponds…
We’ve calculated our instantaneous clearance numbers for the benchtop studies that use sepcon chips. Even calculating per unit area, our chips are not clearing much volume in these experiments. I believe this is due to the very low flow rate (1 µL/min). Kayli is starting experiments to use higher flow rates with the same chips…
Last week, I attended the American Society of Exosomes and Microvesicles (ASEMV) conference in Monterey, Ca and presented a poster on the work that I’ve been doing. Despite a very low turnout for my poster session (due to the scheduling of a long break before and dinner after), I met with some very interesting people…
After the w310 series being beautiful (few pinholes, reasonable strength, high yield), we have retreated somewhat with a return of the copper-related piholes. Here is an SEM image and EDX scan from the well wall: Here is a pic of a pinhole on the membrane with a telltale copper silicide particle attached to the edge:…