Introduction: At the last meeting, I introduced the new stirred cell device acquired in Tom’s lab (here). I have expanded the study to observe changes in flux and concentration for each of the membranes used. Experiments: Each of the experiments was run with BSA having an initial concentration of 1mg/mL, this concentration is determined using spectrophotometry…
Introduction: The lab purchased an Amicon 8010 Stirred Cell which has a 25mm diameter for membranes and holds 10mL of solution. The device works by placing the solution into the cell, pressurizing the system at 8-10 psi, and creates stirring via an elevated stir bar and the stir platform. This type of filtration system was chosen…
Overview: After trying an initial experiment of growing ADSCs on TCP and 3um translucent (TE) membranes and then placing them onto TCP or 3lp SiO2 membranes, it was determined that further studies should be conducted. For this experiment we grew ADSC on TCP, 3um translucent (TE) membranes, and 0.4um transparent (TE) membranes. Once the cells…
Overview: Bob etched 4 non-porous membranes, on the backside of the membrane. HUVECs were to be grown on these etched non-porous SiO2 membranes and compared to unetched non-porous membranes. Below Bob describes how the membranes were etched: “Four of the non-porous SiO2 membranes were placed on a ø6” carrier wafer with the membrane facing up in the…
Overview: The study of ADSC spreading on various surfaces was conducted to determine if porous surfaces and the porosity of the surfaces had any effect on cell spreading. Eight different surfaces were studied and each of the membranes were SiO2, these included: 0.5um low porosity (LP), 0.5um high porosity (HP), 3um LP, 3um HP, 5um…
Overview: The goal of this experiment was to determine which membrane type adipose derived stem cells (ADSCs) would proliferate best on. The ADSCs grew over a four day period and were imaged on days 1, 2, 3, and 4. The cells were counted on each of these days to determine growth rates on each of the…
This paper was found when looking for details on quantifying cell spreading and adhesion. The paper discusses how the group has made mesoporous silicon wafers and their success with growing human umbilical vein endothelial cells on the wafers without additional proteins. DifferentialCellAdhesion
Overview: This experiment was conducted to compare the proliferation of adipose derived stem cells (ADSCs) when grown in different medias. Two medias were used and each had different growth factors and serums. Only 0.5um low porosity membranes were used for this study. Data was collected on days 1, 2, 3, and 6, and was used to compare the…
Overview: This experiment was conducted to determine the difference in human umbilical vein endothelial cell (HUVEC) spreading on different membranes. The cells were grown on the membranes for a specified length of time and then stained and imaged to determine the amount of spread on the membranes. This study was a continuation of HUVEC Spreading on…
Scope: Human umbilical vein endothelial cells (HUVEC) and adipose derived stem cells (ADSC) were co-cultured on SiO2 low density 3um pore membranes. The ADSCs were initially seeded onto the membranes, at 6 hours the membranes were flipped over and placed into a 24 well plate and HUVECs were seeded. Cells were grown for 3 days…