Scope: Adipose derived stem cells were seeded onto Corning 3um tracketch membranes, human endothelial derived stem cells were seeded onto the membranes approximately five hours later. Numerous control wells were also seeded with ADSCs both on membranes and on the bottom of the 24 well plate. Three days after being seeded, media in each of…
Scope: ADSCs were cultured on the bottom of the Corning 0.4um translucent membranes in DMEM+ 10% FBS+ 1% Pen-Strep. After 6 hours, the membranes were flipped over and placed into a 24 well plate. Of the four membranes with ADSCs cultured on them, 2 of them were seeded with HUVECs on the top side of…
Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used as matrices for the cells, the bottom of the plate was used as the control….