SLAS – Society for Laboratory Automation and Screening https://www.slas.org Annual Conference February 6-8, 2017 in Washington DC Attended by Tom Gaborski for informative purposes. Collaborators/colleagues in attendance: Vinay Abhankar (UT-Arlington), Andrea Mazzocchi (alum @ Wake Forest), August Allen (alum @ Recursion Pharmaceuticals) My goal in attending this conference was to learn more about high content…
Membrane pore spacing can modulate endothelial cell- substrate and cell-cell interactions (REVISION) We submitted our “focal adhesions” paper to ACS Biomaterials Science and Engineering late last year. We received reasonable reviewer requests from three reviewers. I think I am mostly finished with revising the figures. Here are the updated figures and my comments on what changed….
Here is a PDF of the presentation I plan to give at BMES this year. The presentation is first an overview of our SiO2 membranes for cell barrier and co-culture models and then a brief summary of our cell-substrate and cell-cell interactions on porous and soft substrates. I am currently working on getting it under…
In our study of endothelial fibronectin fibrillogenesis and FA formation on microporous SiO2 membranes, we decided to investigate whether the pore orientation and pore spacing induced FA formation and therefore F-actin alignment. (Note: we updated our last post on FN fibrillogenesis here.) Spencer and I have been busy analyzing data and making figures. It has…
In our prior work we showed that endothelial cells form far fewer focal adhesions on microporous SiO2 membranes compared to non-porous SiO2 and tissue culture plastic (see prior post by Ana). We performed this analysis at 24 hours, which seems to be the standard in the literature. Interestingly, we found that the number of FAs…
Residual matrix from different separation techniques impacts exosome biological activity (PDF) Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta & Paolo Bergese. Scientific Reports 6, Article number: 23550 (2016) doi:10.1038/srep23550 Abstract Exosomes are gaining a prominent role in research due to their intriguing biology and several…
The purpose of this post is to start a general discussion around the idea of using ultrathin silicon-based membranes and graphene oxide to improve Extracorporeal Liver Support, also called Artificial Liver Support (ALS) for patients with acute liver failure (ALF). What is Extracorporeal Liver Support? This is essentially the equivalent of renal dialysis for the liver….
Based on the talk I gave at the ICNMM (International Conference on Nano, Micro and Minichannels) this past summer, I’ve been asked if I would organize and chair a session titled: Transport in Membranes and Nanofluids. I’d like to include people from NRG as well as membrane collaborators. Consider keeping these dates open: July 10-14, 2016. The conference…
Attached is a paper I’d like to review on Thursday (June 11, 2015). This group is using two planar surfaces in an attempt to mimic a 3D cell culture environment. This is similar to our claims that cells growing on a permeable membrane can be akin to a slice of a 3D matrix. I met…
This is more work that Randi did looking at ADSCs migrating towards HUVEC networks. Motivation: To demonstrate the migration of ADSC toward pre-formed HUVEC networks when ADSC are seeded 4 hours after HUVEC. Method: Monday – Coat 4 wells of a 96 well plate with 100% Geltrex, place in incubator. Seed 20,000 HUVEC cells in…