Here is a PDF of the presentation I plan to give at BMES this year.
The presentation is first an overview of our SiO2 membranes for cell barrier and co-culture models and then a brief summary of our cell-substrate and cell-cell interactions on porous and soft substrates. I am currently working on getting it under 15 minutes.
I got a couple of wafers from Chris last week – 50nm oxide deposited at room temperature or 450 degrees. For each wafer, I saved the zsensor (top), phase (middle) and zsensor (bottom) scans. I also calculated the roughness (the table looking thing on the bottom of each image. I had some problems with imaging…
These are the sieving curves for 50, 75, and 100 nm NPN membranes. The 75 nm NPN 30 nm gold data point is just an estimate, since the membrane broke before enough filtrate could be collected for an absorbance measurement. However, our estimates of zero gold tend to be pretty good (gold is very visible in solution,…
DailyHemo Report of Dr. Fissell’s Implantable Kidney Development Sometimes the link above “Implantable Kidney” doesn’t seem to work. http://www.dailyhemo.org/implantable-kidney
Hey, this will be a rather short post, but kind of important for the current work on my 5637 EV capture. While working over the break, I was messing around with capturing CFSE labeled 5637 EVs and I noticed that there were many large clusters on the membrane surface (Figure 1A). This was concerning to…
Last week, I deposited silicon nitride/ amorphous silicon/ silicon nitride stack and then annealed the free standing stack after etching the substrate. The nitride film was deposited with 25W bias and the silicon film with 5W bias. The thickness for the nitride film is 30nm where it is 25nm for silicon. One of the initial…
In this gel I evaluated the linear range of coomassie brilliant blue. From this test it looks like things are linear from .0156 to .125mg/mL. My guess is that the protein ladder bands in the previous experiment fall within the range. I used the Bradford assay to find the total concentration of the stock protein…