The following contain updates from a group of Swiss collaborators working with NPN for a BBB mimetic. The movies are important because they demonstrate T-cell/endothelial interaction including transmigration and because they show how using vacuum pressure can rapidly cause vacuoles to grow in endothelial cells.
PDMS/membrane deflection was measured on Veeco profiler by JP and me. The main problem with this kind of measurement is that it is taken form the backside of the membrane and the walls need to be excluded from the measurement. Using 20x objective helps but still has some problems. PDMS was first spin-coated on the…
I made a post yesterday showing that in 20mM HEPES, pH7.5, +10mM NaCl solution equivalent amounts of DDM were diffusing through the “treated” and “untreated” SC256 samples. Additionally it appeared that minimal amounts of BSA were diffusing across either sample. I set up another diffusion experiment and let it sit overnight. DDM analysis: As observed…
My original post reported burst pressures on rectangular windows with the chips trench side down in the fixture (pressure from below). These values are low compared to square window pressures. My pressures ranged from 2 psi to 12 psi while square windows achieved 10 psi to 50 psi (Sarah Wayson’s post on square windows). So I did a limited set…
As Greg posted last time, he and I have been working on a manuscript for all of the tomography stuff that we have done that we are going to hopefully submit this week. In that paper, we covered all the tomography techniques that Greg uses to reconstruct models of the pores and then used these…
What happens to cells when they are exposed to a series of bubbles with and without a dubbler? The purpose of this experiment was to prove the hypothesis that bubbles damage cells, and the dubbler prevents cells from experiencing bubble damage. The experiment was performed with a PBS flow rate of about 0.14 mL/min through…
I will keep this post short and start by saying that the process of establishing a reliable set-up for nanosphere deposition, by evaluating the repeatability of the deposition parameters, has been longer than anticipated. The nanospheres self-assemble at the hexane-water interface and stabilize laterally through electrostatic forces. The main deposition steps are: Add water to…