Hello All, I wished to be writing this from some far away tropical resort, as I was told PhD students of the past used to do before they defended their theses. Instead, this comes straight from the ROC homeland, deep in the midst of a global pandemic and a tea-fueled thesis writing session filled with…
Now that I am working on labeling my 5637 EVs, I have finally gotten around to getting a good labeled sample and a good negative control. The samples were prepared as follows: 5637 EVs were thawed from a frozen stock and 1 μL was diluted into 1 mL of labeling fluid. The labeling fluid contained…
Hey, this will be a rather short post, but kind of important for the current work on my 5637 EV capture. While working over the break, I was messing around with capturing CFSE labeled 5637 EVs and I noticed that there were many large clusters on the membrane surface (Figure 1A). This was concerning to…
This is just a quick comparison of the new types of imaging that we now have available to us. As Bill mentioned previously, we have an Andor Dragonfly confocal system now available to us thanks to Patrick Oakes. We have been testing the system extensively and I have been messing around with it for imaging…
To try to calibrate the STED for imaging on our membranes, Bill and I have been trying to use 50 nm silica beads that have been captured on the membrane. However, we discovered initially that the beads were actually not too easy to work with and clumped like crazy. On STED, we weren’t really able…
Silicon nanomembranes are ultrathin, free-standing structures that vary from 10 – 100 nm in thickness. These membranes are at least 1 order of magnitude thinner than the thinnest polymeric membranes. Pores can be manufactured on these membranes to have diameters ranging from 5 nm – 10 μm with porosities ranging from 2 – 20%, allowing…
Hi All, This post is more of a dump of figures that I have put together for Dan’s and my upcoming Journal of Membrane Science manuscript. I have included figures and captions, so any feedback would be greatly appreciated.
This post has been a long time coming, but I have finally (essentially) finished all the work for Aim 1 of my thesis. (Credit to Dan for helping with part of it and getting a thesis along the way, see his post here: https://trace-bmps.org/critical-flux-of-ultrathin-silicon-membranes-in-tangential-flow-filtration-of-protein-solutions/) Up until this point, I have been attempting to validate my…
Okay, so after navigating this weird new method for posting, I have a semi interesting post today. I have been working on the experimental validation of my COMSOL predicted capture results and have finally completed the first round of those experiments (I say this after looking at the data). The experiments have been carried out…
With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have been captured on our membranes. Now, I won’t go fully into the details of how…