The week before last, I finally switched back to my exosome experiments with some hope of repeating my January successes with my new clampable format and two pump system. After failing for several hours (and breaking 7 membranes), I was finally able to get the system to work and get a sample for SEM. (A…
Over the summer, I have been working on a project with Rick Waugh that focuses on separating white blood cells from red blood cells. The goal is to be able to take a lancet droplet of whole blood and perform an analysis on this blood, whether it be for white blood cell phenotyping or exosome…
With changing my channel height to attempt to lower the Peclet number, one important factor that is affected is the transmembrane pressure. Decreasing the channel height will significantly increase the transmembrane pressure, which may stress the membrane and cause it to break. However, in addition to changing the channel height, I will also be changing…
Hi all, So this is slightly different from my previous post, but is perhaps much more important for the work that I am doing. This analysis is important because it allows us to determine the appropriate conditions at which to operate the system. Currently, I operate the device at a flow rate of 10 microliters/minute…
Hey all, So, I’ve been trying to replicate the exosome experiments that I did back during my rotation to varied success. I have had trouble with the plasma that we had frozen with precipitates that would not resuspend upon thawing. We ordered new plasma though so fresh batches of experiments will be coming up very…
Hey all! In this post, I’d like to present some of my final data on the exosome isolation project and cover some data that was generated in response to questions asked during our previous discussion. To begin with, Tom asked whether or not it was possible to measure protein in the filtrate and retentate and…
Hi all, I’m Kilean. I’m a first year grad student in BME doing my rotations. My rotation project has been working on developing a method that can isolate exosomes from blood plasma. We are trying to compete with techniques such as ultracentrifugation and ExoQuick, which are both the current preferred methods for isolating exosomes. …