This will be a very brief post, but kind of an interesting one. I have been attempting to get my identification of exosomes by immunogold labeling and that hasn’t been going terribly well. I think that it is due to a wealth of complications including a lack of fixed exosomes, salt contamination and other problems….
Hey all, I have the first draft of my ASEMV poster here for any feedback that you might have. The conference is in Monterey, CA next week and any feedback that you have on this by Tuesday or Wednesday evening of this week on the poster would be awesome. ASEMV_Poster_KL_09132017_01
One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating in plasma such as cfDNA) can cause problems with identification and characterization of the exosomes….
So, this is the data that we have all been waiting for. Without all the fancy introductions and stuff, I present you with labeled exosomes. They were isolated in tangential flow from my human plasma samples using the SOP that I have been following along with a labeling protocol that Henry and I developed. It…
[latexpage] I know this is my third post today, but this is not a data post. It’s more of a PSA. If you want to add beautiful equations into your post (like I did here) then rather than generating a bunch of images and inserting them into the post, you can simply use . is…
This data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…
[latexpage] Last summer, Tejas and I spent a few days beating our heads against the wall because I was trying to develop a mathematical model of particle capture. Specifically, I was trying to understand what amount of transmembrane pressure we would need to hold a particle on (or in, as we are a politically correct,…
When we are trying to optimize the capture of exosomes in the tangential flow system, it is important to understand how particles are behaving in the flow. Initially, we tried to do this last summer by looking into the particle tracing package for COMSOL, but that required a complete overhaul of the software (a new…
Over the course of this past year and 3 months, I have been on a long and perilous quest to reproduce data that I obtained during my rotation in the McGrath Lab. Specifically, I was looking to capture exosomes in the pores of our membranes, as I showed in my post here (See the last figure…
When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to note is that they typically use 13-15 mL volumes of media to begin their centrifugation…