Beckham Lab Exosome Ultracentrifugation Protocol
When Aslan and I went to the Beckham lab last week, they gave us a copy of their ultracentrifugation protocol for isolating exosomes. I attached it here so that we can have it as a reference. The major thing to note is that they typically use 13-15 mL volumes of media to begin their centrifugation protocol and then they also use a sucrose gradient cushion for the final step. Apparently this whole process takes two days before they get their final exosome product, so we have a lot to work with for our devices.
Where is step 1? Also can you make it a hyperlinked PDF attachment?