Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24 induces BdEC malignancy (turning the normal epithelial cells cancerous). Goal: Investigate if BdEC monolayer will break…
Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24 induces BdEC malignancy (turning the normal epithelial cells cancerous). The ultimately test of malignancy would…
Hi all, recently we improved cell tracking with the use of SiR-DNA label (see highlights below). random migration: A. Representative cell tracking from three independent migration experiments, color-coded by the color bars to the left in red, green, and blue. B. Distributions of motility parameters (persistence time, instantaneous speed, average speed, pathlength, and displacement)…
Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges. Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound healing cells. Recap on angle autocorrelation We look at the correlation of angles between…
Intro/Motivation: SiR-Hoechst is a far-red DNA stain for live-cell micro/nanoscopy and has been shown to be non-cytotoxic. I mentioned to Tom that if we can label the cells we can better automate the cell tracking for faster analysis. Tom knew of this dye and decided to give it a try. This direction subsequently lead to a potential…
Intro Recently we have been collaborating with the Moghaddam lab from the University of Florida, which specializes in the fabrication of graphene oxide (GO) membrane, to study the use GO membrane as a toxin filter or absorbent in hemodialysis and live support devices. We are responsible for the hemocompatibility component of the study and used the following…
[NOTES] BMES 2017 highhlights
Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely that we will have the word”early” from our manuscript title. PS: The old, raw mock…
Hi all, Recently we finished drafting the manuscript titled “Porous substrates promote early endothelial migration at the expense of fibronectin fibrillogenesis.” This manuscript will feature the cover illustrated by Brad (our new medical illustrator in the Gaborski Lab): Long story short (another illustration by Brad): Left (nonporous control substrate): cell traveled slower, covered smaller…
[presentation] HUVEC migration and fibronectin