manuscript mock figures: HUVEC migration and fibronectin

Hello Everyone, As you probably remember, We have been working on optimizing Track Etched membranes devices since last semester, these membranes are 80 nm pore size and our beads are 100 nm in. We have been working on the design to make devices work since the first day of these experiments because of different reasons like…

Here are the results of a protein ladder separation with w677.  If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that never came in contact with membrane, R = Retentate, F = Filtrate Myosin (Band at…

We’ve made a few academic inquiries so far into the theory which governs our nanoporous membrane transfer, but engineers as we are, the pace of our practical knowledge has outstripped that of our theoretical understanding. Here, I want to share a summary of what we know about these transferred membranes from a practical perspective, oriented…

Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes  for migration of FBs and eventually closure (healing) of the wound and second effect of exosomes derived from ADSCs on FBs migration. In order to test our hypothesis, we designed…

I ran some tests today using the 0.01um, 0.05um, and 0.10um pore diameter track-etched membranes from sterilitech. The tool the paul built to cut the membranes works very well, and assembly into the microcon systems it simple. I used the standard vertical-wall o-rings that come in the microcon systems because based on yesterdays test results…