HUVEC spreading on pncSi and glass

Hey, this will be a rather short post, but kind of important for the current work on my 5637 EV capture. While working over the break, I was messing around with capturing CFSE labeled 5637 EVs and I noticed that there were many large clusters on the membrane surface (Figure 1A). This was concerning to…

Six wafers (385-390) were produced last week with the new Solaris 150 RTP system. Good news: We now have much better control/feedback compared to the RIT setup. There are two thermlecouples on the wafer tray that monitors the front and back portion of the wafer. The new unit allows us to independently modify the front/back/bottom…

This post relates to the ongoing project and potential grant between Integrated Nano-Technologies, Simpore, and NRG. The goal of my research is to show that our membranes can be integrated into Nano-Technologies’ device. Our membranes must be able to filter out or hold back large debris that can cause false positives in their device, while…

I briefly experimented with the track-etched membranes this morning and found that they are extremely fragile and near impossible to cut. I disassembled a few of the microcons and found that a circular piece of paper the size of a hole punch (like the one on the bench by the mac) fits perfectly into the…

Since my last update I have been trying to achieve reliable size-separation of DNA by length using dwell time with the NPN pre-filter. Originally I had hoped to achieve 250-bp resolution near the optimal length for the NPN filter devices that we are working with, but that turned out to be a bit overly ambitious,…