Hi all – I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in our NRG meeting. Since these results are confidential, I am not allowed to publish them…
Hi all, I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting
Hello Everyone, Here is a summary of my trip to Nashville to learn about extracellular vesicles in cancer and present my work on isolation of nanoparticles/EVs using ultrathin nanomembranes. Main points of the conference: This conference was about EVs in cancer, and did not include a lot about therapeutic applications of EVs. I could only…
Hi everyone, One of the objectives in the last aim of my PhD is to investigate the effect of soluble factors secreted by adipose derived stem cells (ADSCs) on wound recovery of fibroblasts (FBs). We have categorized these soluble factors to 3 subpopulations including: large-EVs, small-EVs, and non-EV associated molecules. For the last 3 weeks,…
The goal of this set of experiments was to study the growth rate of cells in 3 different media; 1. Complete Media 2. Exosome Free Media 3. Serum Free Media. We studied the effect of media on both ADSCs and FBs. Cells growth was monitored by a Matlab code that Henry has made for us….
Introduction: Fibroblasts: Dermal fibroblasts are cells within the dermis layer of skin which are responsible for generating connective tissue and allowing the skin to recover from injury. A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. Fibroblasts are…
Hello Everyone, This is an update on all my experiments and analysis on PKH paper. As i had a post before about PKH nanoparticles and PKH-Exosome fusion after labelling, we’ve been working on analysis and finishing figures for this paper. Figure 1;Observation of PKH nanoparticles by fluorescent imaging. A and B) Fluorescent images of PKH-labelled…
Hello Everyone, So Shannon and i have been working on studying the effect of exosomes in different conditions for wound healing assays and random migrations. This post is about one of our experiments (wound healing) to give you an update on our data and what we’re able to get with Henry’s matlab code. How the…
Hello Everyone, I’ve been working on exosome labelling and trying to characterize it by NTA. So we read a paper published a month ago, explaining the presence of PKH nanoparticles as false positive results in internalization studies. They studied four different staining protocols and showed the presence of these nanoparticles regardless of the staining protocol….
Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes for migration of FBs and eventually closure (healing) of the wound and second effect of exosomes derived from ADSCs on FBs migration. In order to test our hypothesis, we designed…