PhD Committee Meeting and Internship with Sartorius
Hi all,
I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius.
Best
Hi all,
I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius.
Best
This is a follow-up post to “Protein Assay Test.” I realized that I never posted the final stag of my experiments, in which I finally got BSA to go through the membranes. In “Protein Assay Test,” I described several experiments that I set up to see if I could get BSA to pass through a…
Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is done with Transwells®-style devices. To begin this, we need to review the methods used with…
Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL (1 inch long chip) promotes disruption of laminar flow.
Today while I was imaging a Al2O3 coated pnc-Si membrane on the high-res TEM, I noticed something curious as I placed the beam on a single pore for a few minutes. I was initially interested in the structure of the aluminum oxide coating, but found that something started to deposit on the pore walls as…
In the soon to be submitted paper on diffusion models, we list two contributions to membrane resistance. The first is due to the steric and frictional hindrance a molecule encounters as it enters and diffuses through a pore (Renkin, Deen). The second is due to the time that it takes for the molecule to diffuse…
Last week I conducted a variety of experiments to try to make a nanofluidic transistor (a device described in detail in this previous journal club post). I did some light optimization of the E-beam deposition of Ag films over a 3nm Ti adhesion layer in the PVD lesker machine and found that 12nm of Ag gives…