PETL mixer

Surface functionalization results

With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have been captured on our membranes. Now, I won’t go fully into the details of how…

All of this talk about Zeta Potentials, electro-osmosis and electric double layers got me thinking about these phenomenon in relation to discoloration.  We’ve known that salts accelerate discoloration, and we know that bicarbonate solutions accelerate discoloration as well.  I explained the bicarbonate issue based on the equilibrium between bicarbonate ions and hydroxyl ions (pH).  We…

This blog is to revisit the old TEER data obtained by Barrett from the coculture experiments on pncSi and polymer membranes. The reason behind this analysis is to understand the differences of the raw resistance values obtained for the same cell type but on different membrane configurations. The data is shown below. As we can…

Yesterday I posted results of a diffusion experiment using rhodamine and fluoroscein that showed the pores in SC269 are open both before and after treatment in the UV-Ozone system. I set up another diffusion experiment to test “treated” and “untreated” SC269 samples with diffusion of BSA and DDM. As a reminder the focus of my…

What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure overnight) Ozone Top Electrode + Feet (1 hr, cure overnight) Weaknesses are that there are…