PETL mixer

Scope: Adipose derived stem cells were seeded onto  Corning 3um tracketch membranes, human endothelial derived stem cells were seeded onto the membranes approximately five hours later. Numerous control wells were also seeded with ADSCs both on membranes and on the bottom of the 24 well plate. Three days after being seeded, media in each of…

I’ve recently been exploring hydraulic permeability using the pressure cell.  As you may recall, the majority of our permeability measurements have been done in the centrifuge (EQ format or closed bucket).  The pressure cell allows for a more accurate measurement of permeability because it is not affected by changing water levels or variable driving pressures….

Here is the most up to date figure that I’ve made to compare the simulated and experimental separation results.  I went back through the x-ray crystals and remeasured the longest distances in the molecules.  I used the radii to rerun the simulations and plotted them against protein and rhodamine diffusion data.  Here are the results:…

The movable cutoff gels were more or less successful, but were too dirty for publication (see knowledge page). I’ve been trying to rerun them so that they look nicer, but the darker stain that I used has brought out a few new observations. The first gel following this paragraph is the new gel. The second…