We wanted to prove our previous findings that ADSC crawl toward HUVEC networks and alter them. We did this by using time lapse imagery to demonstrate the migration. We also wanted to show the process of HUVEC network formation. We wanted to use the time lapse imagery to see how quickly ADSC migrate to HUVEC…
In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are attracted to something in the Geltrex causing them to migrate. The ADSC did not migrate…
Our motivation was to look into how ADSC influence HUVEC network formation. My first objective was to investigate the optimal cell count, the best volume of Geltrex and if it was better to use M200 media with or without growth supplements. I then looked into seeding HUVEC on a nonporous membrane. At the same time,…