Booth and Kim (2012) micro-BBB device

Hey all,   So, I’ve been trying to replicate the exosome experiments that I did back during my rotation to varied success. I have had trouble with the plasma that we had frozen with precipitates that would not resuspend upon thawing. We ordered new plasma though so fresh batches of experiments will be coming up very…

In my previous blog, I alluded to the issues I was facing in achieving successful migration of neutrophils through the basement membrane into the collagen gels. I am successfully able to achieve transendothelial migration, but migration beyond the endothelial basement membrane is still not achievable. After a lot of thinking, I realized that this, in…

This post summarizes a quick update on 2 different goals: 1) membrane releasing and 2) SU-8 strain reduction. Here you will find basic background on some of the work that has already been conducted and the planned experimental work that will be conducted in conjunction with Bob Carter and Cody Soule (Co-op student). 1. ZnO as…

I am trying to understand the development of endothelial barrier properties as the cells remodel under the influence of physiological shear stresses. I culture HUVECs inside my device on 100 micron thin type I collagen gel. I coat the gel with fibronectin on top to provide adhesion for the cells. It helps them to stay…

Introduction: At the last meeting, I introduced the new stirred cell device acquired in Tom’s lab (here). I have expanded the study to observe changes in flux and concentration for each of the membranes used. Experiments: Each of the experiments was run with BSA having an initial concentration of 1mg/mL, this concentration is determined using spectrophotometry…

Program C. Gu – Harvard Seminal Experiments Reese and Karnolvsky 1967 Brightman and Reese (1969) Two unique features of CNS endothelium: Specialized tight junctions – Very low rate transcytosis. Tight Junction by EM Ben-Zvi et al 2014 Nature. Transcytosis becomes upregulated not tight junctions. 2-3x injection HRP injection. Tracer containing vessels travel in endothelial cells…