So this is the latest iteration of the thumb-pump device we were working on this summer. As you will see in the diagrams, the idea is to ultimately have saliva go through a filtration step, with a side channel leading to a paper sink for excess/refuse pre-filtrate and a through channel. The latter channel leads…
Within the past few weeks we have been working on finalizing an architecture for PETL dialysis that can be cut out, assembled, and disassembled repetitively with precision. Within the past week we were able to replicate the results Kilean achieved back in March with capturing gold particles in the membrane using tangential two-pump flow. 80…
We are happy to be back this summer. Anthony has been working on setting up a system that mimics Kilean’s dialysis devices. Our approach is different in two important ways. First, we are using PETLs, instead of PDMS/glass. For those who are not familiar with PETLs, here is a short summary: Commercial laminating pouches made…
Hi all, just a quick posting to update you and deposit the current SOP and video instructions for PETLs. Nick has been working this summer on culturing cells on PETLs; its been slow, but we are finding that cells (HEKs) are happy growing on curated (cooked) EVA, not so much on the PET side. Right…
The idea here is to use nano membranes that have been integrated in a chip with the intention to mix otherwise laminar flow streams. It was suggested at one of the meetings that I should superimpose the streams, then have one of them come through the membrane into the other stream. The idea is…
Just wanted to show the detail of PETL channels. A couple of interesting things: The adhesive layer(s) are pretty thick, and look pretty smooth after heat curation. Also, the channels are slightly beveled, probably due to craft cutter blade.
I followed Greg’s example and embedded a broken-membrane chip in a PETL. The idea was to use it as a negative control before I use an intact chip. Laminar flow should be maintained in this scenario in order for us to conclude that the membrane is responsible for mixing, should it occur. 4 layers…
Adding pockets (250 micron long, 76 micron deep) to the bottom of the channel (channel is 500 microns wide, 125 deep) in a 4 layer PETL (1 inch long chip) promotes disruption of laminar flow.
Hi all, a quick update in case you want to know what we are up too. This summer it will be just me at the McGrath lab; the main goal is to produce some preliminary data with an eye on submitting an NSF grant in the fall. The topic is nano-biosensing, using Henry’s shear-free…
Hi All, just a quick post to up date you on what we are up to. 3 Things going on in the lab: Ryan continues to make his nano particles using chips, he is loading them with an anti-inflammatory agent (parthenolide) and testing them on macrophages in vitro. Hopefully more on that soon. Niecy just…