Just wanted to show the detail of PETL channels. A couple of interesting things: The adhesive layer(s) are pretty thick, and look pretty smooth after heat curation. Also, the channels are slightly beveled, probably due to craft cutter blade.
Theoretical molar flow is calculated for membranes with different porosities and is based on data extracted from TEM images using Mike’s program. Experimental data is calculated using air permeability test results. It is associated with porosity only through TEM data. Correlation between two is very good but some points do not agree. One of the…
This post is a brief update on the previous post of HUVEC migration on 0.5 μm porous substrates. Brief Recap We compared cell migration on nonporous (np), 0.5 μm low porosity, and 0.5 μm high porosity SiO2 membranes. Low and high porosity refer to a center-to-center distance of two or three pore diameters, respectively. For…
Hi all, i have to present for a biomechanics journal club meet next Friday (Jun/06/2014) and figured that i might as well double-dip the presentation. Currently i am leaning forward to present this article: Takano (Futai) microfld nanofld 2012 [pocket-sized cell culture] which showcased a pocket-sized, stand-alone device for cell culture. No need for…
I am developing a process for fabricating microporous Parylene Liftoff membranes. I am interested in these as a more flexible substrate for cell culturing. Parylene is relatively strong (compared to say pdms), so it is feasible to work with lifted off pieces of membranes on the order of 1 micron or less in thickness. I list…
Jim and I were wondering if we could “image” the membrane windows with the Tecan. A nice thing about the Tecan is that you can specify the number of measurements per well. For example, in this study, I acquired a 10×10 matrix of fluorescence intensities in a “filled circle” pattern (see below). You can also…
In continuation of work on ultrathin metal electrodes on pnc-Si, I tried changing metal thicknesses at different positions inside the chamber. In this experiment, on the front side of the 30 nm thick membrane the 22 nm metal layer was deposited from three different positions in the same run. The back sides of all the…