WHERE: URMC ** Room # 4-7545 **
WHEN: Tuesday April 16th & Wednesday the 17th.
TIME: 9:00-4:00 P.M.
Please drop off your pipettors early as we expect a good turnout.
Pipettors will be done on a first come, first served basis. Thank you!
Over the last couple of weeks, I’ve been doing confocal fluorescence microscopy over at the Med Ctr. I was hoping this tool would clean up some of the background fluorescence in my immunofluorescent images. More importantly, the confocal can take ‘slices’ of images in the z-plane and stack them on top of each other to…
In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are attracted to something in the Geltrex causing them to migrate. The ADSC did not migrate…
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two time points very few cells were observed on the silicon samples, so we added more…
In my previous post talking about the contact angle measurement of 3-mer coated PEG, there was a lack of adequate controls, and also an unanswered issue of contamination of samples during storage. I would now like to refine the study in more -controlled fashion, and without reusing the old chips. In addition, I am also…
Here is an aggregate of the recent posts/data on the Hemodialysis Project … Device Design/Demo Design idea for assembling multiple chips Gasket design for counter flow dialysis chip Peclet chip demo – > Dialysis Membrane modifications to prevent protein binding (HD PEG) Protein binding is reduced by both Adarza and Shestopolov treatments Damage of pnc-Si by…
Last summer, I attempted to modify the surface of SU-8. The purpose of the experiment was to covalently bond poly(ethylene glycol) (PEG) to SU-8, which would be useful when the SU-8 is used as a scaffold on the membranes. The method of surface modification was derived from the attached article, which can be found here. …