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Spectrophotometer raw data
ByJimUpload just for future reference. This includes the data of KMnO4 serial dilutions absorbance and the stir cell diffusion experiment.
Microporous Magnesium Fluoride Nanomembrane Becomes Stronger as the Film Thickness Increases
The title really says it all. I have been attempting to make a better cell culture substrate with the Magnesium Fluoride Films. Previous posts have shown the overall structure of the film, and now I am trying to quantify the strength with a burst pressure measurement. In order to make a direct comparison, I needed to…
Micropump Update
ByMike HFinally on Friday all the pieces came in that we ordered to connect the peristaltic pump tubing to the 238umOD capillary tubing. This is our first order, after last weeks meeting we ordered a smaller OD capillary tubing (164um) with the same 100umID. I connected all the pieces and attached the micropump to make sure…
New laptop for the TECAN and DLS is finally hooked up
Over the past few days Josh and I installed windows 7, windows office suite, Malvern’s Zetasizer software (available online), Tecan’s I-control software (available in the 3-ring binder in the glass cabinet above the machines), and chrome onto the new(er) laptop in the basement. I copied the files on the old computer that seemed relevant –…
(Lack of) Effects of Anti-CD29 antibody on endothelial barrier function
In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/ This is a small update on the following. CD29 is beta-1 integrins, which are used by neutrophils and endothelial cells to adhere to basement…
Spreading of HUVECs on Dimpled Sylgard-184 Substrates
To analyze the dimpled Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples. We’ve already shown that cells will grow on these membranes in our “Dimpled Sylgard Membrane Device” post and that our substrates are wetting in our “Degassing Experiments”…
