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PEGylation of Coverslips and Chips
I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG Silane in their treatment and half did not (though they were still bathed in toluene…
Wafer 416-T
Wafer 416-T is a TEOS wafer annealed at 900 C. Pinhole density is relatively low. left: (1,0) @ 35kx; right (0,4) @ 35kx left: (1,0) @ 50kx; right (0,4) @ 50kx
Ultracentrifugation Exosome Controls: An Unusual Observation in Separation
ByKileanIn attempting to generate control systems for our exosome work as well as to potentially develop a system that would help some collaborators at the University of Illinois, I have been working with the ultracentrifuge again to hopefully isolate a pure fraction of exosomes, devoid of any protein components. This would allow us to start…
pnc-Si degrades into silicic acid
Over the past month, I’ve posted some results of a colorimetric assay for silicic acid. Previous posts are here and here. These results showed that the assay detected silicic acid after pnc-Si and PSi degraded. However, bare silicon wafers also yielded silicic acid, according to this assay. Since pnc-Si samples have the Si well-side surface,…
Chemical Stability of 30nm Samples with RTP Treatment
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant improvement from sample lifetime of 15nm thick pnc-Si. To see if RTP treatment helped the…
Electrophoretic Cell Update
We were worried that Friday’s experiments were giving us a false positive results. If the electrophoresis breaks the membrane, then we’d expect to see the rhodamine rush through just like it was in our experiments. It’s too hard to view the membranes afterward because they tend to break during removal. I designed some simple tests…
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This belongs in a notebook. Data on the blog does not need to be polished, but it should in a form that communicates a result to others. If everyone uploaded their rawest data, the blog would be unbearable to read.