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Frequency modeling of Ussing chamber (standard) and iBidi chamber
We desire to test the electrical perfomance of our membranes in the iBidi chamber. For comparison, I am using a gold standard Ussing chamber format to examine the contribution of our membranes to the overall frequency response of a system. I am using both a matlab model and a web-simulator (www.circuitlab.com) to examine an equivalent circuit model…
Ar sputt. 15 nm temperature series
Recently, I have been exploring the temperature dependence of post-Si deposition Ar sputtered films (15 nm thick). Below is a comparison of the average pore size, porosity, and density of pores of Ar and non-Ar sputtered films annealed between 800 C and 1100 C. It appears that Ar sputtering increases the avg. diameter and porosity…
Microengineered three-dimensional collagen fiber landscapes with independently tunable anisotropy and directionality
Link to BioRxiv paper The extracellular matrix in vivo is composed of fibers arranged with varying degrees of alignment in different directions. The extent of alignment (anisotropy) and the directionality of fibers are an indicator of breast cancer metastasis, influence T-cell motility, and guide endothelial cell alignment. Similar to the existence of chemical gradients and…
BBB Device V2-8 with Copper Bus Demonstrates Dynamic TEER Reading
I took some copper tape and made a bus bar for BBB device V2-8. I used tweezers to smooth down the surface of the tape on each planar surface, routing the copper to the back side of each face, then collecting the lines to one side of the device. I verified that the connections…
Rotational and translational diffusion of DNA in a nanopore
Small DNA oligomers behave like stiff cylindrical rods. They have both rotational and translational diffusion, and this is of interest when considering how such a rod will diffuse through a nanopore. In this post I briefly consider the effects of rotational and translational diffusion of cylinders of different lengths. The rotational diffusion coefficient can be…
Ultra-Thin Self-Assembled Protein-Polymer Membranes: A New Pore Forming Strategy
I recommend we review this article in NRG or journal club – it was just published online in Advanced Functional Materials this week. I see a lot things in this paper that appear to be motivated by our work. They showed SFM/AFM membrane thickness step height, NP gold separations (and cake formation) and water permeation/permeability. Unfortunately for…
