Hello Everyone, Kilean helped us to set a training session for NanoParticle Tracking Analysis (NTA) in UR Medical Center. For this training session, i had 4 samples including: Beads, Liposomes, Media + 10% serum, and PBS. Here is a summary of what we learned, i am also summarizing a paper that i found for comparing…
Hello Everyone, I have been working with Track Etched membranes since last year and we always had problems with leaking and also not being able to push the flow through the membrane. At the end of the summer, i decided to test my new idea for my devices and finally i have a system which…
Hello Everyone, So as you probably remember, we were able to show the capturing and releasing successfully. In addition, we have a Matlab Code now which helps us for intensity change, so as you can see in the next figure which is the analyzed data for videos at the top, it shows capturing gradually…
Hello Everyone, This is another quick update over our experiments. Exosome Scale Experiments: In all of our experiments, beads are 100nm and pores are 80nm. As you probably remember, in my last post, i talked about hos the membranes are resisstive and they don’t stand these flow rates, so the flow finds another source in…
Hello Everyone, As you probably remember, We have been working on optimizing Track Etched membranes devices since last semester, these membranes are 80 nm pore size and our beads are 100 nm in. We have been working on the design to make devices work since the first day of these experiments because of different reasons like…
Separation of Nanoparticles using surface acoustic radiation force: Surface acoustic waves (SAW) can be defined as mechanical waves which propagate on the surface of an elastic medium which most of its energy is concentrated in the surface. It’s been used in many different biological applications such as separation of cells, tumor cells, microvesicles, nano and…
Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of the project. The cell line that they are working with is T24 with 80 -95% confluency….
Hello Everyone, So first of all, a quick reminder of what i have been working on: we were able to show the trapping of the beads by TE membranes, although in final step and after releasing of the beads, we could still see beads on the membrane, so we hypothesized that the beads are…
Hello Everyone, Following my experiments with Track Etched Membranes: So we came up with this idea to perform SEM imaging. Following are the images that i took for my experiment with 100 nm beads over 100 nm pores after releasing step: So in this image, we can see that there are a lot of beads…
Hello Everyone, I have been working on 100 and 50 nm beads since ACNMM conference to optimize our isolation technique for exosomes and verify that our approach works. Technically, based on the discussion that we had before about Peclet Number, in order to decrease this number, I have been running all my experiments with 1…