Hello Everyone, So Now that we have NanoSight in our lab, i decided to run some experiments with different conditions of media and compare the number of particles in each media. We designed this set of experiments like: Water DMEM DMEM + Exosome Free FBS DMEM + FBS ADSC Conditioned Media HDFB Conditioned Media HUVEC…
[latexpage] Hello Everyone, So i decided to do some calculations on our 2 different sets of experiments (Track Etched and NPN) and compare the applied and the required force of them, in addition to making sure that we are applying enough force on the particles to keep them captured. The experiment conditions for both TE…
Hello Everyone, As you probably know, i have been working on calibration curves for NPN and TE membranes and comparison of these two sets of experiments with each other. This post is all about how i made the calibration curves and how the comparison of TE and NPN experiments would look like. Plotting Calibration Curve…
Figure 2 – Micro scale Experiments with 10 micron fluorescent particles and 8 micron pore size track etched membranes: A) Fluorescent intensity – time plot showing increasing in the intensity signal during the capturing step and decreasing for releasing step. B,C and D) Before capturing, After capturing and After releasing panels, respectively, including schematic images…
Hello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i started working on these experiments, and the first experiment was to capture and release 100…
Hello Everyone, Following of making figures for our first exosome paper, I needed to have SEM images after releasing step. So i ran another experiment and checked the membrane after releasing step. You can see the SEM images of this step: As you’ve probably noticed, compared to after capturing step, you see less captured…
Hello Everyone, Following up the discussion that we had in my last post https://trace-bmps.org/sem-images-for-micro-nano-scale-experiments/about my SEM images after capturing and cleaning. It was suggested to tilt the samples and make sure that beads are inside the pores. So I did a second round of SEM imaging on the same sample. And tilted images clearly…
Hello Everyone, I have been trying to take SEM images in 3 different steps during my experiments with polystyrene beads over Track Etched membranes including before capturing, After capturing and releasing. Micro Scale Experiments: As you might remember, in Micro-scale experiments, we use 10 micron beads and 8 micron pores. Before capturing: This is only…
Hello Everyone, Please note that Figures are all shown in the powerpoint file. The goal of these experiments was to plot a standard curve for concentration and intensity in my Track Etched Membranes. In order to that, we decided to dry droplets on the membranes with different dilution, then check the fluorescent intensity of them…
Hello Everyone, So following up on my exosome scale experiment and my last post, i am presenting my last update to summarize my results and to show that my results are trustable and reproducible. This is how we take images: We have different positions in the interaction area, and we take images as the experiment…