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iBidi Microfluidic Integration Progress
As part of my work over at the University of Nottingham, I’ve been using iBidi devices to grow cells. Eventually, I will use their product to do Raman studies of cells grown on my MgF2 substrates. I’ve been making some progress towards that end using PDMS (I’ve been using 10:1 base to curing agent for…
Fabricating 500 nm Channels in Silicon Wafers
Summary This post complements Fabricating 0.5 um Channels in Microscope Slides for Staph Invasion Assays. Challenges in the etch transfer process necessitated switching to a different substrate that had stronger selectivity. With that in mind, we set out to create 500×500 nm channels in silicon wafers for the bone invasion study. We had partial success (micron-sized…
NPN membrane: Streaming potential measurements
ByJirachaiUpdate: After doing several measurements again, it appears that the data shown below were not repeatable. It is believed that these data were just the artifact. Please see my new blog post “Flow Sensing and Energy Harvesting Capabilities of NPN Membrane” for a new data set. Here are the streaming potential measurement data for NPN membrane…
Charged Based Protein Separations
Separations were performed at 2 different salt concentrations with equal volume filtrate and retentate (to avoid dilution problem). Separation time was 40hrs rather than the usual 24. The lower salt concentration run has a lower cutoff – which is just like what we see in the DNA separations. Remember though that proteins have different charges…
PES protein separations
This was the first try at using PES for protein separations by diffusion. 100kD cutoff Nanoseps were used to separate 10mM KCl and 100mM KCl standards. In both cases, the backside fluid was gone after 24hours. I don’t think evaporation was the problem; either the membrane sucked up the fluid or it was drawn up…
Solid- State Nanopores Integrated with Nanoporous Membranes for Enhanced Single-Molecule Counting of Low-Abundance Biomarkers
ByJimWe are putting the final touches on a submission to NIH based on our work with Vincent Tabard-Cossa in Ottawa. I want to provide everyone with a small overview. The basic premise is that solid nanopores (ssNPs) are the right tool for detecting low abundance biomarkers for personalized medicine and point-of-care diagnostics. This premise follows…