Confocal Analysis of Apical and Basal ICAM-1 Expression Following Polarized Cytokine Delivery

ByAlec
Fig. 1 | Abluminal PMN dynamics following polarized cytokine stimulation. a, Probability density functions of perivascular PMN speeds. n = 30, 3 independent devices, 10 PMNs per device. b, Spider plots of PMN tracks and mean and median persistence values. c, PMN tracks closely representing the population mean persistence. Red represents the PMN migration pattern (path length) and blue represents the PMN displacement over 30 min.
Fig. 2 | Confocal analysis of EC ICAM-1 expression. a, 3D reconstruction of confocal stacks depicting apical and basal ICAM-1 expression following polarized cytokine stimulation. Yellow arrows emphasize ICAM-1 rich ridges present above PMN nuclei. devices, 2 cells per device, representative images shown. b, ICAM-1 label integrated density in the nuclear region as marked by DAPI. c, ICAM-1 label integrated density to the periphery of the nucleus. Integrated densities were normalized to max values for clarity. Plots represent mean values at each z-stack height (0.49 μm increments, 8 μm total height). 0 μm represents nanoporous membrane focus.
Movie 1 | ICAM-1, Luminal TNF-α
Movie 2 | ICAM-1, Abluminal TNF-α
Fig. 3 | RhoG regulates endothelial apical cup assembly downstream from ICAM-1 engagement and is involved in leukocyte trans-endothelial migration. The Journal of Cell Biology 2007

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