I recommend we review this article in NRG or journal club – it was just published online in Advanced Functional Materials this week. I see a lot things in this paper that appear to be motivated by our work. They showed SFM/AFM membrane thickness step height, NP gold separations (and cake formation) and water permeation/permeability. Unfortunately for…
Overview I burst pressure tested TEM chips with 1×1 mm windows of 100 nm SiO2 that were both annealed and unannealed (provided by JP and Josh Miller). In our experience it seems that the forming gas (H2/N2) annealed chips are stronger and never wrinkle. These burst pressure tests were performed in an attempt to…
Over the weekend, I resubmitted our adult Adipose-Derived Stem Cell (ADSC) paper titled: Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions There were three main findings: 1.) ADSCs can be induced to express CD31 (PECAM-1) like endothelial cells. a.) Several prior studies used Vascular Endothelial Growth Factor (VEGF) at 50 ng/mL to…
This is a recent paper in Nature Materials that shows stem cells are influenced by prior culture on varying stiffness substrates. This means that when cells are induced towards adipogenic or osteogenic lineages, their prior culture history on a stiff or soft substrate affects their future differentiation. This is further evidence that in vitro culture of stem…
In conducting experiments to address reviewer comments, we again created endothelial-like cells from adipose derived stem cells (ADSCs). As in the past, the vast majority of ADSCs express some CD31 (PECAM-1) after culture in endothelial media (M200) supplemented with 50ng/mL VEGF (on 0.5LP, 3.0LP and TCP). The expression of this CD31 is relatively low and…
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future post we will quantify the degree of spreading at 1hr, 4hrs and 24 hrs. Calculation…
We still need help with NanoDays http://doodle.com/paq66krxtf7mt6tk There are two time slots: 10:45am-2pm 1:45-4pm Please wear your I Love Nano shirts from last year. I have a couple more size Medium and one size Small. The Rochester Museum & Science Center is on the corner of Goodman and East Ave.
PLEASE read this journal article for NRG on Tuesday March 18th @ RIT (PDF links are at the bottom) Surface Chemical Modification of Poly(dimethylsiloxane) for the Enhanced Adhesion and Proliferation of Mesenchymal Stem Cells Shreyas Kuddannaya,†,§ Yon Jin Chuah,‡,§ Min Hui Adeline Lee,‡ Nishanth V. Menon,‡ Yuejun Kang,*,‡and Yilei Zhang*,† I’d like to discuss this paper…
Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions Submitted to Journal of Cellular and Molecular Bioengineering (a BMES journal) ADSC-All-Figures FIGURE 1. Ultrathin microporous silicon dioxide (SiO2) membranes. (A) 300 nm thick SiO2 membranes were fabricated on silicon wafers and etched into 5.4 x 5.4 mm dies with 2 x 2…
Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells. Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…