Mechanical memory and dosing influence stem cell fate

Introduction Proliferation, invasion and metastasis of cancer cells require bidirectional cell-cell communication. Cell-cell communication involves the secretion of oncoproteins, mRNAs, RNAs and DNA fragments by cancer cells. The particles that get secreted by these cancer cells are encapsulated in extracellular vesicles (EVs), which include exosomes. Since evidence suggests that cancer cells secrete more EVs than…

I worked with Paul this afternoon and finally we highly suspect the raw material used for the prototype diffusion chamber could be Polycarbonate. Paul could not provide direct evidence for the material source since he did not keep any purchase orders or materials data sheet… However, we did check our chamber with another plastic board…

Along with differently sized square membranes, I also designed a wafer with different sizes of slits.  On each chip, there is a 4000, 2000, 1000 and 500um long window.  The thicknesses of these windows are 200, 100, 50 and 25um.  I’m interested to see if there is an aspect ratio that causes endothelial cell alignment/tubulogenesis. …

Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test both factors concurrently we designed experiments in an attempt to capture 1.24 um diameter fluorescent…

Hello everyone! this post describes how we are going about determining the size cut-off of the membranes we make through nanosphere lithography. These are 120 nm thick silicon nitride membranes with controlled pore size between 320 and 80 nm. A polymeric scaffold is placed on them to increase robustness. The scaffold is a square grid…

Process Summary for the week of 12/3 -12/10/2007: Simpore Process Flow.xls JP