Journal Club for June 6. Voting is Now Open.
Voting is open until NRG tomorrow. We’ll critically discuss the winner and summarize the 2nd place paper.
[yop_poll id=”3″]
Voting is open until NRG tomorrow. We’ll critically discuss the winner and summarize the 2nd place paper.
[yop_poll id=”3″]
Background: The human Tendon-on-Chip (hToC) spans collaboration between the Center for Musculoskeletal Research (CMSR) at the UR and the McGrath lab to develop an in vitro platform to study inflammation, fibrosis, and cell-cycle regulation in fibrovascular tendon healing. Interaction between the blood-tendon-barrier (BTB) is fundamental to the investigation of this pathology as part of the…
I recently tested intact samples from W415-T and W416-T. All samples from W415-T were completely intact except the one located 3.167 units from center, which had one medium pinhole. All samples from W416-T were intact, but a few had irregular membrane shape, one with constant area and two with about +5% membrane area. I thought…
So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation of albumin and cytochrome c via diffusion. 50 uL of 1 mg/mL BSA and cytochrome…
Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab. We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a height image of the pnc-Si chip with the well side down. Notice the slight decrease…
Jirachi has been working to make thin nafion films using our membranes as a support structure. Nafion is polymer that contains a sulfonate group, and as a result acts as a proton wire, allowing for cations (+) to be transported across a membrane, but not anions or electrons. Such a membrane can be used for filtering a…
This is the protocol I use to measure band intensities. At the end of the explanation I will post and review my recent results concerning silver stain densitometry. First load a digitized gel into ImageJ Select Image>Type>8 bit to make it black and white. Select Invert to make the bands have positive intensities. Go to…