Partial Sieving Curves for 50 and 100 nm Thick NPN

Over the past few weeks, I have been trying to separate proteins with the SepCons. As a preliminary test, Barrett and I decided to first acertain that BSA could pass through a membrane with pinholes. Theoretically, the pinholes are so large that the BSA should easily pass through. However, it doesn’t appear that the BSA…

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as the seeding density.  I always had 3 samples – bEnd3 alone, NG10815 alone and the…

I’ve been using a Makerbot 2 for the last week or so, trying to see if we can use the same PDMS contact lithography techniques that we are all familiar with. In short, yes, but there is plenty of work to be done to make it a robust process for microfluidics.   Makerbot Printer The…

In this post I look at our membrane resistance and compare it to the best performing alumina membrane. Here is the resistance figure I made for the paper: This figure shows the device resistance with and without a membrane.  The membrane resistance would be the difference between the white and grey bars.  We would like…

As a continuation of the substrate bias series, I deposited a 15 nm film with 05 W substrate bias and annealed at 1000 C. TEM shows that this condition generates a 13.4% porosity and a max pore size of ~40 nm! Here is a comparison of all the bias conditions tested thus far: I believe…

In my last post (the first co-culture), I showed that bEnd3 cells exhibited a different morphology when they were co-cultured.  I also decided that I would reverse the orientation of the co-culture in order to better visualize the endothelial barrier.  That is, grow bEnd3 on the basolateral side and NG10815 on the apical side of…