I wish I had read Tucker’s lat post before trying this, but since I just got the steam jig from the machine shop I wanted to try it out. My mistake in the two transfers I attempted was that I applied the steam evenly as opposed to the suggested 45 degree angle, but the results…
I spent some time testing the various steps of the transfer and wetting process to see if I could find the cause of membrane delamination that I saw earlier. The result seems to be that time is the main culprit. Here is the membrane just after transfer (dry between the membranes initially, steam after transfer…
My first attempt at a nanopore fabrication/DNA experiment in NPN assemblies was interesting but ultimately unsuccessful. As I said in our last meeting, I have been able to reproduce the membrane transfer process (using some steam between the two membranes to make good contact) and the wetting behavior using a freezer to condense vapour on…