I assembled two 2-chip dialyzers using the thicker, 75-nm NPN membrane chips (10 mm long multichannel, 22 mm x 24 mm). The dialyzers were pumped full of IPA, to fill with fewer bubbles, then infused with PBS for at least one hour. Unfortunately both of these either broke when hooked up to the animal, or…
Upper and lower gaskets used to make a counter flow device. The danger is that the enclosing pdms can easily flex into the membranes on the flat side of the chip. A hard plastic cover can be used to prevent accidental contact between the PDMS and membrane.
The pump’s data sheet (located here) describes the pumps materials as: polyphenylene sulphone (PPSU) polyimide (PI) nitrile butadiene rubber (NBR) It also says that the acceptable particle tolerance is < 50 μm. This is the info we will need to determine the types of particles we can use with an in-line flow system.
Just wanted to post an update on the creation of a counter flow system using the pncSi chips from wafer 619 (the wafer with the etched trenches for the insertion of capillary tubing). Over the past few weeks Henry and I have been able to produce a few functional counter flow systems using UV Ozone…