Mike and I tried to pass 3 um beads mixed with rhodamine through the stir cell to prove that, if the membrane we intact, only the rhodamine would pass through and not the beads. As seen in the pictures below, only the rhodamine passed through, not the nanoparticles, since these particles are 100x larger than…
I ran a few tests with rhodamine to see if the fluorescence will transfer across the cell. The problem I keep having is that the stir cell set-up is very sensitive. If the wing nuts are tightened too much the membrane will break. But if they are too loose the liquids will leak out. Luckily…
Mike and I tested the movement of Rhodamine between the two cell channels to prove that the Rhodamine particles do diffuse through the membrane. After inspection in the microscope we saw that the fluorescence from the Rhodamine does transfer from one channel to the other across the membrane. However, we also noticed that the Rhodamine…