Author: Alan

To determine whether cell proliferation is a function of membrane porosity and/or pore size, human umbilical vein endothelial cells (HUVECs) were cultured on various surfaces.  The cells were seeded on low and high porosity membranes of 0.5 um and 3.0 um pore sizes.  Non-porous membranes served as control membranes.  All membranes were coated with Geltrex…

The goal of the experiment was to use a fluorescent dye to label live cells in order to measure proliferation over several days.  To avoid cell toxicity, the intensity of the fluorescence was reduced to ~1.5%.  Human umbilical vein endothelial cells (HUVECs) were seeded in a a 96-well plate at a density of 5000 cells…