This is an update to my last post, where HUVECs mixed with collagen inside PDMS channels seemed to stay rounded up and not really do much of anything exciting. This time around I made a few adjustments: New collagen protocol – gives 1.5 mg/mL solution before mixing with cells: -10% 10x PBS -1.4% NaOH -38.6%…
Michael and I have been working to create cell channels of varying widths and heights to test out a concept used in a paper we recently read as a group. This work claims to see cell cords in 12 hours, so no media renewal is necessary. The channels were made by layering and bonding…
The following diagram lays out each separate setup that was tested using different combinations of Geltrex and substrates. Each trial has a different reasoning behind why it was done. The purpose of these trials was to investigate the effects of Gelrex gel and PCTE membrane inserts on tube formation in Bend.3 cells. First, we showed…