This initial aim of this project was to obtain “pockets” with sub-100nm pores, while the top opening is much larger. Dr. Carter is the mastermind behind this project. The following simple fabrication process diagram explains the current approach. After initial failed experiments for obtaining very small openings, Marcela and I aimed for larger bead sizes…
Continuing the studies on neutrophil transmigration in devices with embedded parylene membranes of different pore sizes and thicknesses, three pore sizes (1.0 um, 1.4 um and 1.7 um) and two thicknesses (0.7 um and 1.2 um) were used for these sets of experiments. Thickness ratio of 1.2 um to 0.7 um roughly correlated with…
Tom came up with this idea of using these membrane with precise thickness and pore size control to obtain a critical thickness and pore size, beyond which neutrophils cannot go transmigrate. N-Formylmethionyl-leucyl-phenylalanine (fMLP) was used in the lower channel to serve as a chemotactic factor for transmigration. Additionally, for membranes with pore sizes or thicknesses…
Parylene-C is a biocompatible, inert, FDA-approved and nondegradable material, which can be used to fabricate mechanically robust thin membranes. Parylene is used in the medical device industry for coating implantable devices and in the microelectronics industry as insulator. Furthermore, it has been used in biomedical research for a wide variety of applications. In addition, parylene-C…
Adeel and I summarized our key takeaways from the BMES in this post. I personally tried to attend organ on a chip talks and posters, which included a micro- or nano-porous membrane or a micro/nano-structured co-culture system. I tried to identify weaknesses and strengths to highlight our potential opportunities to contribute, and to improve our…
As the first phase of my thesis, I have been working on parylene C membranes as potential easy-to-make thin polymeric membranes for blood brain barrier models following Dr. Carter’s works. The initial goal is to create membranes with different thicknesses with a reliable technique. Method First, the wafers are coated with micro-90 as a sacrificial…