Author: Zachary Oppito

When the HUVECs were first brought up, the first test for me to perform was to see how they reacted to CMFDA and repeated fluorescent excitations.  Based on the implications from previous research (summarized here), there is the possibility of harm or phototoxicity from the dye.  Since HUVECs are more valuable and ‘fragile’ than either…

Invitrogen offers a number of stains and dyes for use in tracking cells and fluorescent imaging of tissue culture.  One prevalent live stain is called CMFDA (5-Chloromethylfluorescein Diacetate), a green fluorescent molecular probe.  This stain is utilized for tracking cells and monitoring growth & viability of the cells. While performing studies using CMFDA, there appears…