Similar Posts
BSA Retention in Single Channel Dialyzer
Regarding the single channel dialyzer chip with 20-nm thick nano-porous silicon membrane: 10 mm long, 300 µm deep, 500 µm wide channels. Now that we’ve shown that a measurable quantity of urea is filtered out of DI water in one pass through the single channel dialyzer chip, we turn our attention to “Things Which Must…
ADSC Proliferation on Various SiO2 Membranes
ByAndreaOverview: The goal of this experiment was to determine which membrane type adipose derived stem cells (ADSCs) would proliferate best on. The ADSCs grew over a four day period and were imaged on days 1, 2, 3, and 4. The cells were counted on each of these days to determine growth rates on each of the…
Electrospinning aligned polymeric fibers on parylene stencils: cell culture and pll-g-PEG coating update
A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given that there is a parallel project with membrane masking of ECM with parylene stencils, we…
ASAIO notes
ASAIO /əˈsī ō/ noun American Society of Artificial Internal Organs. There is an associated Journal, “ASAIO Journal”, and a yearly conference for the presentation and discussion of research relating to advances in medical device technology. While the initial focus of the society was on renal devices, the artificial kidney, currently the majority of work by…
COMSOL model of SiMPore Liftoff device
I drew an approximation of the SiMPore liftoff device and ran the COMSOL model with 1 mL/min urea fluid flow in the upper channels and 2 mL/min dialysate in the bottom channels. With this initial model, the urea was cleared to 40% (60% removed). This is an instantaneous urea clearance rate of K= 0.6 mL/min….
Less is More: Lower Initial Bacteria OD Increases Transmission in uSiM-CA Bacteria Assays
On our quest to optimize bacteria assays using µSiM-CAs, we recently discovered that higher initial bacterial ODs decrease transmission in positive controls, described here. This was something we did not initially anticipate, engendering a new hypothesis around the idea that less initial bacteria leads to more transmission across our membranes in the µSiM-CAs due to…
