Booth and Kim (2012) micro-BBB device
Here is the paper I reviewed at the NRG meeting on 06/19.
I’ve also included my presentation:
This week I’m working on designing the basal and apical chambers, considering the necessary shear stresses.
Here is the paper I reviewed at the NRG meeting on 06/19.
I’ve also included my presentation:
This week I’m working on designing the basal and apical chambers, considering the necessary shear stresses.
Based on last week’s lab meeting, I made a “solution array” to test discoloration. Specifically, I made 3 solutions of NaHCO3 (0, .23125, .4625, .925 mg/mL) and 3 solutions of NaCl (0.1, 0.5, 1 M). I placed these non-RTP’ed w648 chips in the oven at 37C and took pictures at 4, 18, 72 and 120…
Because of Harold Smith’s project, Rachel and I have been working on various methods to get proteins through a pnc-Si membrane and onto a polymer membrane. The particular system that Dr. Smith wants to study is very sensitive to ambient RNAses, molecules that digest RNA. This means that the long times required for a diffusion…
HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with FBS. Hence, media w/o serum can be used as a possible control for the growth…
I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells. Here’s a post of images. I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …
Today I looked at a wafer (391) that had been annealed in the RTP on top of a 6″ wafer substrate. The idea is that the six inch substrate would create a “shadow” around the 4″ wafer which would improve thermal uniformity by blocking the multiple reflections from the lamps inside the chamber. Here is…
I did a quick experiment in order to show everyone what the Tecan allows us to do. I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an absorbance scan and found that the maximum absorbance was at 530nm (panel A). Then I…