Booth and Kim (2012) micro-BBB device
Here is the paper I reviewed at the NRG meeting on 06/19.
I’ve also included my presentation:
This week I’m working on designing the basal and apical chambers, considering the necessary shear stresses.
Here is the paper I reviewed at the NRG meeting on 06/19.
I’ve also included my presentation:
This week I’m working on designing the basal and apical chambers, considering the necessary shear stresses.
I want to verify that I can grow a confluent monolayer of cells on my MgF2 nanomembranes. I have tried to do this in the past, but have only gotten sparse cells overall before the membranes ruptured. I believe that part of the problem was that I was not seeding at a very high density…
We’ve got pores! Unfortunately, we are still being plagued by an unknown contamination that seems to be “eating” away at our membranes. This would explain why we’ve been having a hard time separating. We also observe a strong radial dependence in porosity AND size with these RT deposited films. In the past it was limited…
In a previous post, we showed that both Cytochrome C and BSA at concentrations of ~1mg/mL through 20nm cutoff Sepcons with about the same filtrate concentration (~60% of the feed concentration). Yesterday (1/14/13) we repeated these experiments with 10 and 15nm cutoff Sepcons and demonstrated pretty clearly that either 10 or 15nm Sepcons can be used…
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below. After 86 hours the cells…
Along with differently sized square membranes, I also designed a wafer with different sizes of slits. On each chip, there is a 4000, 2000, 1000 and 500um long window. The thicknesses of these windows are 200, 100, 50 and 25um. I’m interested to see if there is an aspect ratio that causes endothelial cell alignment/tubulogenesis. …
In order to figure out the relationship between grain size and pore size, I tried to use XRD measurement to determine the grain size by Scherrer equation. t = kλ/(B cosθ) where t is the average grain size, k is the shape factor that is in the range 0.87-1.0 (it is usually assumed to be…