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Strutting the Runway: Modeling the Microfluidics and Reaction Kinetics of the μSiM-DX
The development of the μSiM-DX has been swift and focused, as discussed in these previous blog posts: https://trace-bmps.org/beadazzled-laying-the-foundation-of-the-%ce%bcsim-dx-fouling-based-sensor/ and https://trace-bmps.org/vindictive-virus-capture-testing-the-%ce%bcsim-dx-with-vaccinia-virus-towards-a-modular-virus-diagnostic/. To provide a greater understanding of the inner workings of the device, I have done a few calculations regarding some of its working properties related to microfluidics. The three I present here are about the relationship…
HD Membrane Stability tests: 4 hour Water and PBS
This post is from Xuan ‘Sabrina’ Pan Purpose The nanomembrane used in my experiments is expected to promote efficiency of hemodialysis (HD) for end-stage renal disease patients. The membranes, however, broke during HD for rats, and breaking membranes can lead to unwanted consequences. My task is to figure out the transmembrane pressure during dialysis under…
ADSC differentiation and networking in various environments
ByAndreaScope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used as matrices for the cells, the bottom of the plate was used as the control….
PEGylation of Coverslips and Chips
I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG Silane in their treatment and half did not (though they were still bathed in toluene…
We have the Tecan M200 until Oct. 6th!
Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th. Please come try out your assays on it. Or check sensitivities. Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…
Transport through 'non-porous' membranes
There’s been lots of discussion about “non-porous” membranes and the possibility that they are actually porous. By “non-porous”, I mean that a TEM of that membrane doesn’t have obvious pores by pore processing/our eyes. This could mean a couple things: either the pores are so small that they are hard to resolve by TEM or…