Lab tours on Friday
Prospective Graduate Students will be touring the lab this Friday from 12:30-1:00. Please be working in the lab and ready to engage!
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BSA diffusion across SC267
ByMike HLast week I made a post showing detection of detergent diffusion across membrane SC267. In the diffusion set up I had placed 6uL of a 50/50 volume ratio solution of 1.0% DDM and 1mg/mL BSA with 60uL of DI-water on the membranes backside. I allowed the setup to sit for two days and then used…
Implications of Hemodialysis Modeling for Large Animal Studies
Down the road, we’re going to move towards large-animal studies to test our hemodialyzers, since a large animal model (either a sheep or a pig, preferably the former) allows us to model the effects of complete kidney failure (as opposed to just uremia in rats) and work on a scale that would be comparable to…
Multi-annealing of free standing OSO stack (2)
ByJoe QiLast time I showed that the sample with multi-annealing yielded higher porosity and larger average pore diameter than the sample with direct annealing. In this post, I further investigate the multi-annealing of free standing OSO stack (20/15/10) by varying the pre-annealing time. The heat treatment process is illustrated in the following figure. Free standing OSO…
Modeling Oxygen and Urea transfer in Nanomembrane Devices
Introduction Extracorporeal membrane oxygenation (ECMO) devices are used to supply oxygen to patients when they are unable to get sufficient oxygen through their lungs. Miniaturizing an ECMO device could be beneficial for neonatal patients, as it would require less blood to prime the device and operate efficiently at a lower overall flow volume. Dialysis is…
CAD-LB: Colocalization Detection Guide
Introduction In this post I will review work I have completed to further characterize the CAD-LB platform. In this set of experiments combinations of nanoparticle species were used to define colocalization limitations. More specifically, two nanoparticle species each labeled with a unique fluorescent dye (red or blue) were used to simulate pan EV and antibody…
No edge effect with NG10815 cells
I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells. Here’s a post of images. I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …