BBB Device V2-8 with vias through bulk silicon

“Dynamic Particle Manipulation in Microfluidic Devices Employing Dielectrophoresis” Dr. Blanca H. Lapizco-Encinas Associate Professor Microscale Bioseparations Laboratory Chemical and Biomedical Engineering Department Rochester Institute of Technology Tuesday, February 26, 2013 8:30 am River Campus Robert B. Goergen Hall Sloan Auditorium, Room 101

Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips.  “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test if a brief (~1 hour) exposure to very high pH causes discoloration.  DMEM+10%FBS without the…

We finally achieved a separation using the EQ format. 4 SepCons from wafer 319 were loaded with 500 uL of .93 mg/mL clarified brain extract. They were run at 1000 rpm (which we roughly figured to be 3.3 psi initially) for 2 hours. After 2 hours, I judged the flow through the membrane to be…

A couple months ago, I tried to use tetramethylrhodamine (TAMRA) as a fluid-phase marker to label vacuoles.  The idea is that the only way this stain could be internalized is if it is pinocytosed, eventually ending up in vacuoles.  If you follow this link, the dye worked OK but not great.  I found a couple…

In our study of endothelial fibronectin fibrillogenesis and FA formation on microporous SiO2 membranes, we decided to investigate whether the pore orientation and pore spacing induced FA formation and therefore F-actin alignment. (Note: we updated our last post on FN fibrillogenesis here.) Spencer and I have been busy analyzing data and making figures. It has…

  Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells.   Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…