The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside walls of the pore.
I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with 3 micron spacing, and 20% porosity for this experiment. Microporous membranes can facilitate neutrophil transmigration,…
After all the nafion-coated pnc-Si chips that Jirachai gave me broke during my last attempt to filter salt water, Jirachai was kind enough to supply me with a new batch of chips, this one a mix of SiN and pnc-Si, both covered in a thin film of nafion. The idea was that since Nafion is…
I’ve tested several membrane types with the streaming potential apparatus in order to calculate zeta potential. Here are the results: All materials in the above chart have negative zeta potentials. Pnc-Si seems to have the highest overall potential, followed by PES then cellulose. Cellulose is prone to cracking and bursting at pressure above 7 PSI….
Hello Everyone, Here is an update of our meeting in Strong Hospital: So They are working in an urology lab, We met Sam who is a technician in Dr. Carla Beckham’s lab, he is responsible for cell culture part of the project. The cell line that they are working with is T24 with 80 -95% confluency….
We created a 25-40-25 OSO stack with a SEPCON pattern. RTP at 1050 C 100 C/s 60 s. The following images show a comparison between the STEM detector and the SEM detector, and then a couple more images at 100 kx and 50 kx. I calculated a porosity of ~6.5 %, and…
I’ve upgraded the WordPress publisher to version 2.6. The most useful change is the ability to track changes to any post/page, compare revisions, and revert to an older version. Here’s a video showing the changes: https://videopress.com/v/mARhRBcT/fmt_std Additionally, I’ve installed some plug-ins that should make posting and keeping up with new posts easier: There is now…
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The brightness of the image may even be significant. Was the beam noticeably dimmer with the thick PVP coating. IF so, the film is very thick.
Anyway, the pores are clearly covered, so the fact that water passes so easily must mean that the film either dissolves or is highly porous. It will be interesting to see if there is an effect on separations.