The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside walls of the pore.
Our motivation was to look into how ADSC influence HUVEC network formation. My first objective was to investigate the optimal cell count, the best volume of Geltrex and if it was better to use M200 media with or without growth supplements. I then looked into seeding HUVEC on a nonporous membrane. At the same time,…
Since I am posting data that is acquired using Auger electron spectroscopy, I thought that it might be a good idea to write a post about the theory of the instrument, to better understand why this data is useful and relevant. The Auger effect is a phenomenon in which an atom that is excited by…
We have been collaborating with Britta Engelhardt’s group at the University of Bern in Switzerland since Tejas and I met them at the CNS Barriers conference in Paris in 2015. They are interested in studying live T-cell transmigration across human BBB models as part of their research program in Multiple Sclerosis (MS). Prior to working…
I asked Tom to add gold PES separations of NPs to his earlier figure and to evaluate the loss of NPs during the use of these filters compared to pnc-Si. Here is what he came up … All experiments (including pnc-Si) were done at 10 PSI with 200 ul of starting material above the membrane…
Last week I took a series of TEM images from all but one position on wafer 638. Below are the TEMs, histograms, and porosities vs. distance from center plots. “Xpos”, “xneg”, “ypos”, “yneg” indicate which axis the images were taken from. Positive x-axis Negative x-axis Positive y-axis Negative y-axis Wafer 638 porosity vs. position While…
160112 LO Grant Update
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The brightness of the image may even be significant. Was the beam noticeably dimmer with the thick PVP coating. IF so, the film is very thick.
Anyway, the pores are clearly covered, so the fact that water passes so easily must mean that the film either dissolves or is highly porous. It will be interesting to see if there is an effect on separations.