The unwashed membrane has a definite “film” over it which makes the pores look textured. The MeOH & H2O seems to clear the pores up, but there are some places where there is remaining PVP that sticks to the inside walls of the pore.
The ultimate experimental goal of the desalting project is the removal of salts from a salt/protein solution while fractionating certain sized proteins with maximum protein retention and recovery.Thus far we have characterized the flux of salt through our membranes which show flux an order of magnitude greater than cellulose membranes. Our next step is to…
Parallel with my efforts in the Osteomyelitis project to coat our membranes with polyurethane acrylate, I began working with the Gill lab on improving the performance of our uSiM platform as a propagation assay for Staphylococcus aureus. In the past we have gotten a lot of good data using this assay, but its performance has…
I derived the governing equation for the volume transfer from SepCon devices in the wet/wet (EQ) format. It is … where the equilibrium volume for the cup can be calculated apriori using the cross sectional areas of the cup and the surrounding annulus … with the total volume of interest given by and the time…
Recently, several studies on Staphylococcus aureus (S. aureus) have been hindered due to poor performance from assays using the µSiM-Canalicular Array (CA). As discussed here, we attempted to improve assay performance by coating our membranes with fibronectin. This did not work well and instead left us in a state of confusion as we did not…
Introduction In addition to in situ measurements of small molecule permeability (Parts 1, 2 and 3), we wish to enable an assessment of permeability by sampling from the abluminal chamber of the µSiM in a manner similar to what is done with Transwells®-style devices. To begin this, we need to review the methods used with…
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
Comments are closed.
The brightness of the image may even be significant. Was the beam noticeably dimmer with the thick PVP coating. IF so, the film is very thick.
Anyway, the pores are clearly covered, so the fact that water passes so easily must mean that the film either dissolves or is highly porous. It will be interesting to see if there is an effect on separations.